SECM4 Specimen Preparation Workshop
October 27-29, 2025, Florida State University
The 2025 SECM4 specimen preparation workshop will focus on conventional cryo-EM specimen preparation and preparation for difficult samples. Topics covered will include principles of vitrification, hands on cryo-EM specimen preparation, preparation of specialized grids, and more.
Agenda
Full schedule TDB
Day 1 - Negative staining and screening
Participants will perform negative staining and screening on a TEM, and perform conventional cryo-EM specimen preparation
Day 2 - Specialized grid preparation
Participants make specialized grids and learn techniques for overcoming challenges with difficult specimens
Day 3 - Dealing with difficult samples
Participants will screen their cryo-EM grids and learn how to process data for difficult samples.
Venue and Travel information
Venue
BioUnit I
89 Chieftan Way
Tallahassee, FL 32306
Hotel
Accepted workshop applicants will have rooms reserved for them at a nearby hotel. The workshop will cover the cost of the hotel room for accepted applicants.
Travel and Meals
Participants are responsible for covering their own travel. Meals will not be provided but there are many nearby and affordable locations for meals. The Seminole Cafe is a very short walk from the venue and offers breakfast, lunch, and dinner at a reasonable price.